Novi podaci o vrsti Pinctada radiata (Leach, 1814) (Bivalvia: Pteriidae) u Jadranskom moru

The first recorded population of Pinctada radiata in the Adriatic Sea was observed on the pier in Porto Montenegro marina (Tivat, Montenegro), in August 2016. Numerous individuals inhabited pier walls at a depth of 5 m. The shell height (SH) values for the 15 randomly collected individuals of P. radiata ranged between 32.2 mm and 52.1 mm with an average SH value of 38.3 mm (standard deviation ±6.1). Further surveys in Montenegro will provide information on the establishment of the observed population and what is its impact on local biodiversity.Prvi nalazi populacije Pinctada radiata u Jadranskom moru se odnose na dokove marine Porto Montenegro (Tivat, Crna Gora), u kolovozu 2016. godine. Na zidovima dokova zabilježene su brojne jedinke na dubini od 5m. Visina ljušture (SH) za 15 slučajno odabranih jedinki P. radiata je kolebala od 32.2 mm do 52.1 mm sa srednjom vrijednošću od 38.3 mm (stand. dev. ±6.1). Daljna istraživanja u Crnoj Gori će omogućiti informacije o uspješnosti uspostavljanja ove populacije i njenom uticaju na lokalnu bioraznolikost

Prvi nalaz endemskog žarnjaka Spinimuricea klavereni (Carpine & Grasshoff 1975) (Cnidaria, Anthozoa, Plexauridae) u Jadranskom moru

We present the first record of the endemic Mediterranean anthozoan Spinimuricea klavereni (Carpine & Grasshoff 1975) (Cnidaria, Anthozoa, Plexauridae) for the Adriatic Sea (Boka Kotorska bay, Montenegro). A density of 0.72 colonies m-2 could be measured in the area, which was com-pared with the scant available data in the literature. Having in mind that this species is very rare and endemic for the Mediterranean, it is important to increase our knowledge on its distribution and biology in order to better assess its ecological role, the pending threats and the need for specific conservation measures.Prikazan je prvi nalaz endemičnog sredozemnog žarnjaka Spinimuricea klavereni (Carpine & Grasshoff 1975) (Cnidaria, Anthozoa, Plekauridae) za Jadransko more (Bokokotorski zaliv, Crna Gora). Dobijeni rezultati i izmjerena gustina od 0,72 kolonije m-2 su uspoređeni s oskudnim podacima dostupnim u literaturi. Imajući u vidu da je ova vrsta vrlo rijetka i endemska za Sredozemno more, važno je poboljšati znanje o njenoj distribuciji i biologiji kako bismo bolje procijenili njenu ekološku ulogu, prijetnje i potrebu za određenim mjerama zaštite

Učinci pridnenog koćarenja na bentoske zajednice u južnom Jadranu (Crna Gora)

The purpose of this study is to show the effects of bottom trawling on the benthic assemblages in the south Adriatic Sea as well as to report detailed quantitative and qualitative data on some invertebrate groups of no commercial value that are affected by trawling. Short-term effects of bottom trawling on the soft bottom seafloor were studied on the continental shelf and upper slope in the southeastern Adriatic Sea. Ten sites were trawled in July 2011. A total of 14,069 invertebrate organisms belonging to 44 taxa were collected within the study period. Of these, 93 were Sponges (4 species), 509 Cnidarians (7 species), 3,670 Molluscs (5 species), 48 Bryozoa (1 species), 3,154 Echinoderms (14 species), and 7,054 Tunicates (13 species). Cluster analysis performed with the data from different depth layers showed two clearly separated main groups that corresponded to shelf and slope zone. The shelf zone samples were characterized by higher species richness, while samples taken from the slope contained one species, Pteroeides spinosums. The obtained results showed that the most common species during the survey were Pteria hirundo, Ascidia virginea, Phallusia mammillata, Botryllus schlosseri, and Centrostephanus longispinus. Species P. mammillata, B. schlosseri, and P. regalis presented the highest value of biomass index (kg/km2). Our results showed that ascidians, cnidarians, and echinoderms are the most vulnerable taxa to trawling.Cilj ovog istraživanja je prikazati koje učinke pridneno koćarenje ima na bentoske zajednice u južnom Jadranu, kao i izvijestiti o detaljnim kvantitativnim i kvalitativnim podacima o grupama beskralješnjaka koji nemaju komercijalnu vrijednost, a koje su pogođene koćarenjem. Kratkoročni učinci pridnenog koćarenja na mekano morsko dno proučavali su se na epikontinentalnom pojasu i gornjem nagibu u jugoistočnom dijelu Jadranskog mora. Tijekom srpnja 2011. koćarilo se na deset lokacija. Tijekom razdoblja istraživanja prikupljeno je ukupno 14069 jedinki beskralježnjaka koji pripadaju u 44 svojte od čega su 93 spužve (4 vrste), 509 žarnjaka (7 vrsta), 3670 mekušaca (5 vrsta), 48 mahovnjaka (1 vrsta) 3154 bodljikaša (14 vrsta) i 7054 plaštenjaka (13 vrsta). Cluster analiza, koja je provedena s podacima prikupljenim na različitim dubinama, pokazala je da postoje dvije jasno razdvojene grupe koje pripadaju padini i nagibu. Uzorci prikupljeni u padinskoj zoni bili su bogatiji vrstama, dok je u uzorcima prikupljenima na nagibu pronađena samo jedna vrsta, Pteroeides spinosums. Iz prikupljenih uzoraka zaključili smo da su sljedeće vrste bile najučestalije: Pteria hirundo, Ascidia virginea, Phallusia mammillata, Botryllus schlosseri, i Centrostephanus longispinus. Kod vrsta P. mammillata, B. schlosseri, i P. regalis, uočena je najviša vrijednost indeksa biomase (kg/km2). Naši rezultati pokazali su da su svojte organizama poput mješčićnica, žarnjaka i bodljikaša najugroženije koćarenjem

Fucus spiralis extract and fractions: Anticancer and pharmacological potentials

Purpose: Sea macroalgae are an important source of biologically highly valuable compounds. The main aim of this study was to investigate the in vitro anticancer properties and chemical composition of the dichloromethane-methanol extract and three fractions of the Fucus spiralis from coastline of Morocco. Methods: Fractions were made from dichloromethane: methanol (1:1) extract of Fucus spiralis: petroleum-ether, ethyl-acetate and n-butanol. Extract and fractions were screened for in vitro cytotoxicity by MTT assay against human cervical adenocarcinoma (HeLa), colorectal adenocarcinoma (LS-174T), lung carcinoma (A549), and normal human lung fibroblasts (MRC-5). Cell cycle distribution of the HeLa cells was evaluated using flow cytometry. Acridine orange (AO)-ethidium bromide (EB) staining was used to assess morphological changes of HeLa cells under fluorescence microscope. Anti-migration and anti-angiogenic properties were investigated using scratch and tube formation assays against human endothelium-derived permanent EA.hy926 cell line. Antidiabetic activity was tested using anti-α-glucosidase assay. Antimicrobial effect was tested using micro- dilution method. Results: Petroleum-ether fraction оf Fucus spiralis rich in fatty acids exerted the highest cytotoxicity against HeLa cells. Ethyl-acetate and petroleum-ether fractions induced the highest accumulation of the HeLa cells in sub-G1 and G2/M phases. Extract and fractions showed proapoptotic effect on HeLa cells under fluorescent microscope. They exhibited antimigratory and antiangiogenic effects in vitro. IC50 value for α-glucosidase inhibitory activity was much stronger than standard acarbose. n-Butanol fraction exerted the highest antibacterial and antifungal activity. Conclusions: The investigation of various biological activities of the extract and fractions obtained from Fucus spiralis may suggest a promising anticancer and pharmacological potential of this edible macroalga

New Mediterranean Biodiversity Records (November 2018)

In the present article, new records are given for 18 species (6 native, 9 alien and 3 cryptogenic), belonging to 5 Phyla (i.e. Chlorophyta, Cnidaria, Annelida, Mollusca and Chordata), from 8 Mediterranean countries: Spain: A large population of Polycerella emertoni reported from the Ebro Delta, the presence of pinimuricea klavereni is confirmed for the first time in the Catalan coast; Italy: the new record of Cryptonome turcica extends its distribution to the western Mediterranean Sea, the findings of Mawia benovici constitute the southernmost record of this species in the Adriatic Sea; Slovenia: second record of the alien seaslug Cuthona perca in the Mediterranean Sea; Montenegro: Styela plicata communities were recorded in Boka Kotorska Bay; Albania: the native great torpedo ray Tetronarce nobiliana recorded for the first time in Albanian waters; Greece: first record of Lagocephalus sceleratus in the Greek side of the Adriatic, the records of Cassiopea andromeda and Pterois miles are first records of these species from the Ionian Sea, the colonial ascidians Aplidium coeruleum and Didemnum protectum are recorded for the first time in the Eastern Mediterranean, an extremely abundant population of Melibe viridis is found in Messolonghi lagoon, the record of Synchiropus sechellensis is the range expansion of this species in Greek Seas, Acetabularia caliculus is recorded for the first time from Greece; Cyprus: first records of Callionymus filamentosus and Haminoea cyanomarginata from the island; Turkey: the presence of Berthellina citrina in the Mediterranean is confirmed.info:eu-repo/semantics/publishedVersio

Unpublished Mediterranean and Black Sea records of marine alien, cryptogenic, and neonative species

To enrich spatio-temporal information on the distribution of alien, cryptogenic, and neonative species in the Mediterranean and the Black Sea, a collective effort by 173 marine scientists was made to provide unpublished records and make them open access to the scientific community. Through this effort, we collected and harmonized a dataset of 12,649 records. It includes 247 taxa, of which 217 are Animalia, 25 Plantae and 5 Chromista, from 23 countries surrounding the Mediterranean and the Black Sea. Chordata was the most abundant taxonomic group, followed by Arthropoda, Mollusca, and Annelida. In terms of species records, Siganus luridus, Siganus rivulatus, Saurida lessepsianus, Pterois miles, Upeneus moluccensis, Charybdis (Archias) longicollis, and Caulerpa cylindracea were the most numerous. The temporal distribution of the records ranges from 1973 to 2022, with 44% of the records in 2020–2021. Lethrinus borbonicus is reported for the first time in the Mediterranean Sea, while Pomatoschistus quagga, Caulerpa cylindracea, Grateloupia turuturu, and Misophria pallida are first records for the Black Sea; Kapraunia schneideri is recorded for the second time in the Mediterranean and for the first time in Israel; Prionospio depauperata and Pseudonereis anomala are reported for the first time from the Sea of Marmara. Many first country records are also included, namely: Amathia verticillata (Montenegro), Ampithoe valida (Italy), Antithamnion amphigeneum (Greece), Clavelina oblonga (Tunisia and Slovenia), Dendostrea cf. folium (Syria), Epinephelus fasciatus (Tunisia), Ganonema farinosum (Montenegro), Macrorhynchia philippina (Tunisia), Marenzelleria neglecta (Romania), Paratapes textilis (Tunisia), and Botrylloides diegensis (Tunisia).Stelios Katsanevakis, Michail Ragkousis, Maria Sini, Markos Digenis and Vasilis Gerovasileiou were supported by the Hellenic Foundation for Research and Innovation (HFRI) under the “First Call for HFRI Research Projects to support Faculty members and Researchers and the procurement of high-cost research equipment grant” (Project ALAS – “ALiens in the Aegean – a Sea under siege” (Katsanevakis et al. 2020b); Project Number: HFRI-FM17-1597). Konstantinos Tsirintanis was co-financed by Greece and the European Union (European Social Fund-ESF) through the Operational Programme “Human Resources Development, Education and Lifelong Learning”, 2014-2020, in the context of the Act “Enhancing Human Resources Research Potential by undertaking a Doctoral Research” Sub-action 2: IKY Scholarship Programme for PhD candidates in the Greek Universities. Maria Zotou was supported by the project “Coastal Environment Observatory and Risk Management in Island Regions AEGIS+” (MIS 5047038), implemented within the Operational Programme “Competitiveness, Entrepreneurship and Innovation” (NSRF 2014-2020), co financed by the Hellenic Government (Ministry of Development and Investments) and the European Union (European Regional Development Fund, Cohesion Fund). Razy Hoffman was supported by Yad-Hanadiv Foundation, through the Israel Society of Ecology and Environmental Sciences and Israel Nature and Parks Authority, an integrated program for establishing biological baselines and monitoring protocols for marine reserves in the Israeli Mediterranean Sea (Grant #10669). Tatiana Begun, Adrian Teaca and Mihaela Muresan were supported by the European Union’s Horizon 2020 BRIDGE-BS project under grant agreement no. 101000240. Fiona Tomas was supported by the project “Invasion of the tropical alga Halimeda incrassata in the Balearic Islands: ecology and invasion dynamics (AAEE119/2017)”, funded by the Vicepresidencia y Consejería de Innovación, Investigación y Turismo del Govern de les Illes Balears, with support from the European Union and FEDER funds, and the project “Una nueva alga invasora en el Mediterráneo: invasibilidad, detección y erradicación del alga tropical Halimeda incrassata (INVHALI)”, funded by the Fundación Biodiversidad, del Ministerio para la Transición Ecológica y el Reto Demográfico. Simonetta Fraschetti, Laura Tamburello, Antonia Chiarore were supported by the project PO FEAMP 2014-2020 - DRD n. 35/2019, “Innovazione, sviluppo e sostenibilità nel settore della pesca e dell'acquacoltura per la Regione Campania” (ISSPA 2.51) and the EU EASME - EMFF (Sustainable Blue Econ-omy) Project AFRIMED (http://afrimed-project.eu/, grant agreement N. 789059). Carlos Jimenez, Louis Hadjioannou, Vasilis Resaikos, Valentina Fossati, Magdalene Papatheodoulou, and Antonis Petrou were supported by MedPan Small Projects, Mava, and LIFE-IP. Louis Hadjioannou, Manos L. Moraitis and Neophytos Agrotis received funding from the European Union’s Horizon 2020 research and innovation program within the framework of the CMMI/MaRITeC-X project under grant agreement No. 857586. Ernesto Azzurro was supported by the project USEIt - Utilizzo di Sinergie operative per la gestione integrata specie aliene Invasive in Italia, funded by the research programme @CNR. Antonietta Rosso and Francesco Sciuto were supported by the University of Catania through “PiaCeRi-Piano Incentivi per la Ricerca di Ateneo 2020–22 linea di intervento 2.” This is the Catania Paleoecological Research Group contribution n. 484. Diego K. Kersting was supported by the Beatriu de Pinós programme funded by the Secretary of Universities and Research (Government of Catalonia) and the Horizon 2020 programme of research and innovation of the European Union under the Marie Sklodowska-Curie grant agreement No 801370. Francesco Tiralongo was supported by the AlienFish project of Ente Fauna Marina Mediterranea (Scientific Organization for Research and Conservation of Marine Biodiversity, 96012 Avola, Italy), a citizen science project for monitoring and studying rare and non-indigenous fish in Italian waters. Adriana Vella, was supported by funds through the BioCon_Innovate Research Excellence Grant from the University of Malta awarded to her. Noel Vella was supported by REACH HIGH Scholars Programme-Post Doctoral Grant for the FINS project. Some of the records provided by Victor Surugiu were obtained during surveys carried out within the framework of the project “Adequate management of invasive species in Romania, in accordance with EU Regulation 1143/2014 on the prevention and management of the introduction and spread of invasive alien species”, SMIS 2014+ 120008, coordinated by the Romanian Ministry of Environment, Water and Forests in partnership with the University of Bucharest (2018–2022). Alan Deidun and Alessio Marrone were supported by the “Spot The Alien” citizen science campaign for the monitoring of the Alien species in the Maltese archipelago and by the Interreg Italia-Malta Harmony project. The authors from the National Institute of Biology (Slovenia) acknowledge the financial support of the Slovenian Research Agency (Research Core Funding No. P1-0237) and of the Ministry of Agriculture, Forestry and Food (project “Survey of the species richness and abundance of alien species in the Slovenian Sea”). Emanuele Mancini and Fabio Collepardo Coccia were supported by the project PO-FEAMP 2014-2020 “BIOBLITZ: research, knowledge and participation for the sustainable management of marine resources (BioBlitz Blu 2020)” coordinated by CURSA for MIPAAF, the Italian Ministry of Agricultural, Food and Forestry Policies, Measure 1.40 - Protection and restoration of biodiversity and marine ecosystems and compensation schemes in the context of sustainable fishing activities. Daniele Grech was supported by the PO-FEAMP 2014-2020 project ECOGESTOCK “Approccio ECOsistemico per la tutela e la GEStione delle risorse biologiche e STOCK ittici nelle acque interne”, the citizen science project Progetto Fucales: chi le ha viste? and the Paralenz Every dive counts sponsor. Jamila Rizgalla was supported by the project Snowball for the monitoring of alien species in Libyan waters له اهتفش له اهتدطصا ؟) have you seen it have you fished it?). Gerasimos Kondylatos and Dimitrios Mavrouleas were supported by the project “EXPLIAS” (MIS (ΟΠΣ): 5049912), design and piloting methods of commercial exploitation of invasive alien species with a view to contributing to their population control, coordinated by the National Technical University of Athens with the collaboration of the Hellenic Centre for Marine Research and the University of the Aegean and co-founded by Greece and the European Union. G. Kondylatos and Savvas Nikolidakis were supported by the project “SAMOS” (ID CODE: 32.2072004/001), a study for a submarine productive park in Marathokampos of Samos. Paraskevi K. Karachle, Aikaterini Dogrammatzi, Giorgos A. Apostolopoulos, Kassiani Konida and Melina Nalmpanti were supported by the project “4ALIEN: Biology and the potential economic exploitation of four alien species in the Hellenic Seas”, funded by NRSF 2017-2020 (MIS (ΟΠΣ): 5049511). Fabio Crocetta and Riccardo Virgili were partially funded by the project PO FEAMP Campania 2014–2020, DRD n. 35 of 15th March 2018, Innovazione, sviluppo e sostenibilità nel settore della pesca e dell’acquacoltura per la regione Campania, Misura 2.51, WP5, Task 5.5 Presenza e distribuzione di specie non indigene del macrozoobenthos e del necton in Campania. Michel Bariche was partially funded by the University Research Board of the American University of Beirut (DDF 103951/2592). Constantinos G. Georgiadis, Dimitra Lida Rammou, Paschalis Papadamakis and Sotiris Orfanidis were supported by the MSFD monitoring program. Sonia Smeraldo was supported by the MPA-Engage project, led by the Institute of Marine Sciences of the Spanish National Research Council and funded by the Interreg MED program. Evgeniia Karpova acknowledge that the publication of this article was in part carried out within the framework of the state assignment of the FRC IBSS “Patterns of Formation and Anthropogenic Transformation of Biodiversity and Bioresources of the Azov– Black Sea Basin and Other Regions of the World Ocean” (No. 121030100028-0). Elena Slynko’s work was carried out within the framework of a State Assignment no. 121051100109-1 of IBIW RAS. Manuela Falautano and Luca Castriota were supported by ISPRA citizen science campaigns for the monitoring of alien species through the dedicated institutional project ([email protected]). María Altamirano was supported by the project RUGULOPTERYX funded by Fundación Biodiversidad-Ministerio para la Transición Ecológica y el reto Demográfico (Spain) and the project UMA20-FEDERJA-006 with support from the European Union and FEDER funds and Junta de Andalucía. Records provided by L. Mangialajo were collected in the framework of projects funded by the Pew Charitable Trust, by the European Commission (AFRIMED, http://afrimed-project.eu/, grant agreement N. 789059) and by the Académie 3 de l’Université Côte d’Azur (projet CONVOST).Peer reviewe

Unpublished Mediterranean and Black Sea records of marine alien, cryptogenic, and neonative species

To enrich spatio-temporal information on the distribution of alien, cryptogenic, and neonative species in the Mediterranean and the Black Sea, a collective effort by 173 marine scientists was made to provide unpublished records and make them open access to the scientific community. Through this effort, we collected and harmonized a dataset of 12,649 records. It includes 247 taxa, of which 217 are Animalia, 25 Plantae and 5 Chromista, from 23 countries surrounding the Mediterranean and the Black Sea. Chordata was the most abundant taxonomic group, followed by Arthropoda, Mollusca, and Annelida. In terms of species records, Siganus luridus, Siganus rivulatus, Saurida lessepsianus, Pterois miles, Upeneus moluccensis, Charybdis (Archias) longicollis, and Caulerpa cylindracea were the most numerous. The temporal distribution of the records ranges from 1973 to 2022, with 44% of the records in 2020–2021. Lethrinus borbonicus is reported for the first time in the Mediterranean Sea, while Pomatoschistus quagga, Caulerpa cylindracea, Grateloupia turuturu, and Misophria pallida are first records for the Black Sea; Kapraunia schneideri is recorded for the second time in the Mediterranean and for the first time in Israel; Prionospio depauperata and Pseudonereis anomala are reported for the first time from the Sea of Marmara. Many first country records are also included, namely: Amathia verticillata (Montenegro), Ampithoe valida (Italy), Antithamnion amphigeneum (Greece), Clavelina oblonga (Tunisia and Slovenia), Dendostrea cf. folium (Syria), Epinephelus fasciatus (Tunisia), Ganonema farinosum (Montenegro), Macrorhynchia philippina (Tunisia), Marenzelleria neglecta (Romania), Paratapes textilis (Tunisia), and Botrylloides diegensis (Tunisia).peer-reviewe

New data on Aplidium tabarquense (Tunicata: Ascidiacea) distribution in the Adriatic Sea

The colonial ascidian Aplidium tabarquense Ramos-Esplá 1991 was recorded in Montenegro during a monitory survey carried out by scuba divers on an underwater outfall of the wastewater discharge system in TraÅ¡te Bay. Several colonies were present on artificial substrata at a depth of 27 m. This is the first record of the species in the South Adriatic Sea. Further observations are recommended both to delineate the effective distribution and population density of A. tabarquense in the Mediterranean Sea and to widen the knowledge on ascidian diversity along Montenegroâ\u80\u99s coasts